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Affinity Measurement ServiceIntroduction

KMD Bioscience has been dedicated to the study of interactions between biomolecules for many years. Research on the interactions of nucleic acids, proteins, viruses, and other biomolecules, and understanding their mechanisms of action and functional mechanisms, is a hot topic in current life science research. Affinity is an important parameter for determining the strength of interactions between molecules, and it is a crucial indicator for understanding molecular functions, drug discovery, and screening. Our scientists, with years of experience in interaction research and testing, can provide high-quality Affinity Measurement Services. We ensure precise control of every experimental step to guarantee the smooth progression of customer experiments.

Affinity is a characteristic parameter for evaluating the strength of interactions between two molecules during a reversible reaction, making it an essential indicator for molecular identification, biological processes, drug discovery, and screening. It holds significant importance in early-stage drug research, screening and identification, as well as downstream production quality control. Moreover, it has broad applications in life science basic research and biopharmaceutical development. Based on our comprehensive platform, our team is committed to offering high-quality, one-stop technical services to meet the various experimental needs of our clients.


亲和力测定英文站Affinity determination.png

Types of Affinity Measurement Services

We offer accurate and rapid affinity measurement services based on platforms like Octet and Biacore. We provide a variety of molecular interaction analysis services tailored to customer needs, including but not limited to commonly used detection technologies: Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI). Our services ensure accurate, objective, and reliable measurement results. We are committed to offering high-quality, one-stop technical services to meet the various experimental needs of our clients.

BLI Affinity Measurement: This is an optical analysis technique that measures changes in the thickness of the surface layer of a biomolecule fixed on a biosensor. When a target molecule interacts with the fixed molecule at the sensor’s surface, the change in quantity causes a corresponding change in the interference pattern, which is used to obtain information about the molecular interaction.

SPR Affinity Measurement: This method involves fixing one molecule on the surface of a sensor chip, while continuously flowing another molecule in solution over the chip. The detector can monitor the binding and dissociation of molecules from the chip’s surface in real-time. SPR records changes in the mass of molecules on the sensor chip's surface, providing real-time monitoring of molecular interactions.

Competitive ELISA Method for Measuring Affinity: A simple method for affinity measurement. Antibodies are mixed with antigens at different concentrations in an aqueous solution to reach equilibrium. Unbound antibodies are captured by antigens coated on a microplate and detected through the ELISA method.

Sample requirements

Sample Type

Requirements

Antibody, Protein, Peptide, Small Molecule Compounds, etc.

* Buffer:PBS, HEPPS, etc., free of organic reagents, without Tris, EDTA or DDT;

* Protein Samples (including antibodies):200 µg, concentration >0.5 mg/ml;

* Peptide Samples:200 µg, concentration >1 mg/ml;

* Small Molecule Compounds:1 mg, concentration >1 mg/ml, purity >90%, dissolved in 100% DMSO or water.

Service advantages

-- Diverse detection platforms, meeting different testing needs.

-- High-accuracy analysis instruments, experienced operations, and strict quality control, ensuring reliable and accurate results.

-- High detection efficiency, no labeling required, enabling high-throughput testing with real-time monitoring.

-- Simplified method design, easy to operate and flexible.

-- Higher instrument sensitivity, with lower sample consumption.

-- Strong anti-interference ability, detection unaffected by external factors such as temperature, refractive index of buffers, etc.

-- Wide application range, suitable for protein, nucleic acid, peptide, and nanomaterial molecular interaction analysis.

-- One-stop service experience, offering integrated solutions from affinity measurement to molecular interaction studies.

Recommended Services

Our team, relying on advanced platforms such as Octet and Biacore, provides customers with fast and precise Affinity Measurement Services. Based on customer-specific needs, we offer various molecular interaction analysis options, including but not limited to common Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI) techniques. Our services are designed to ensure the accuracy, objectivity, and reliability of test results. Through these technologies, we provide high-quality one-stop technical support, meeting different experimental needs and helping researchers achieve better results in fields such as molecular interactions, drug screening, and antibody optimization.

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