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Murine Single B Cell mAb Production
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Murine Single B Cell mAb ProductionIntroduction

 KMD Bioscience specializes in recombinant monoclonal antibody (mAb) preparation with extensive experience in multi-species systems. Our validated workflows and GMP-capable facilities deliver premium services for rabbit, mouse, camel, sheep, and chicken-derived recombinant mAbs.


Mouse systems offer accelerated breeding cycles, compact housing requirements, and well-characterized immune responses across validated strains. Mouse monoclonal antibodies (mAbs) demonstrate high target specificity, minimal lot-to-lot variation, and typically negligible background cross-reactivity. Single B-cell Antibody Production Technology provides accelerated timelines, high-throughput capacity, and enhanced specificity compared to traditional methods. KMD  Bioscience utilizes that technology to perform direct PCR amplification of antibody genes from isolated antigen-specific B cells, followed by sequencing, expression vector construction, expression validation, and purification in an integrated workflow. This enables production of high-specificity, high-affinity mAbs within condensed timelines


Advantages of Single B Cell Antibody Development

Category

Single B Cell Technology

Hybridoma Technology

Phage Display Technology

Antibody

Natural

Natural

Unnatural

Antibody Affinity

High

High

Medium

Druggability

High

High

Low

Turnaround Time

16–20 weeks; Optional rapid immunization

20–26 weeks; Optional rapid immunization

16–20 weeks; Optional rapid immunization

Antibody Gene Sequence

Obtain directly

Further sequencing required

Obtain directly

Antibody Diversity

High

Medium

Medium

Species Available for Screening

Unrestricted species

Mouse, Rabbit

Unrestricted species

Mouse Single B Cell Monoclonal Antibody Development Technology

KMD Bioscience's Single B Cell mAb Production Platform is based on Fluorescence-Activated Cell Sorting. Leveraging the principle that each B cell produces only one specific antibody, it directly amplifies antibody genes from individual B cells and obtains specific antibodies through recombinant expression. By employing single B cell technology, antigen-specific B cells can be obtained directly from peripheral blood after animal immunization, and the antibody gene sequences are cloned. The resulting murine monoclonal antibodies exhibit outstanding advantages, including high specificity, high activity, and high affinity. 

To meet diverse client needs, KMD Bioscience provides comprehensive, one-stop technical services encompassing antigen design, synthesis, and modification, through animal immunization, single B cell enrichment and screening, expression and purification, to functional validation.

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Single B Cell Monoclonal Antibody Development Service

Step

Service Content

Delivery Materials

Timeline

Isolation of Animal Immune PBMCs

* Immunize the mice 4-5 times
* ELISA titer determination
* Peripheral blood collection
* PBMC isolation

* ELISA test results
* Standard experimental record
* PBMC, 1×106 cells per 10 vials

4 to 10 weeks
(Either standard immunization or rapid immunization can be selected)

Fluorescence-Activated Cell Sorting

* PBMC Fluorescence-Activated Cell Sorting

* Standard experimental result record

1-2 business days

Single B Cell Culturing and Screening

* 5 to 20 plates of 96-well plates for single B-cell culture and Positive clone screening

* Positive clone screening results
* Standard experimental record

2-3 weeks
(Additional validation methods are available)

Positive Clones Sequencing

* At least 20 positive clones underwent RT-PCR and sequencing

* RT-PCR results
* Original sequencing data
Specific sequence
* Standard experimental record

1 week

Vector Construction for Antibody Expression and Purification

* Construction of mammalian expression vectors
* Cell transfection
* ELISA verification

* At least 3 positive clone sequences
* Expression vector
* Purified antibody: 500 Micrograms per positive clone
* ELISA test results
* Standard experimental report

5-6 weeks
(Additional validation methods are available)


Sample Requirements

Customer Provides

Requirements

Recombinant Proteins

* ≥2.5 mg protein: Purity >85%, molecular weight >10 kDa. Soluble protein concentration: 0.5~5 mg/mL. For antigen affinity purification: Additional 10 mg recombinant protein required.

Peptides & Small Molecules

* ≥10 mg unconjugated peptide: Purity >90%, ≥10 amino acids. Requires conjugation with KLH, BSA, OVA, or other carrier protein.
* Small molecule: Requires pre-delivery structural evaluation.

Advantages of Single B-Cell Monoclonal Antibody Development Services

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