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Antibody Sequencing ServiceIntroduction

KMD Bioscience has been dedicated to antibody research for many years. Monoclonal antibodies play a crucial role in drug development and diagnostic reagent development. The primary structure of the antibody molecule, especially the amino acid sequence of its CDR regions, is central to its biological function. Accurately and rapidly analyzing its complete sequence is of great significance. Based on high-resolution mass spectrometry and high-fidelity gene cloning technology, we offer high-quality Antibody Sequencing Services for our clients.

We have a skilled team of experimental technicians with extensive experience in antibody sequencing, allowing us to deliver high-quality sequencing results within a short timeframe. We utilize high-quality Orbitrap Fusion Lumos mass spectrometers and the fully-equipped antibody sequencing data processing software (PEAKS AB Software.) By combining gene sequencing with bioinformatics databases, we have built a Next-Generation Antibody Sequencing Platform capable of quickly and accurately analyzing the antibody’s primary structure, ensuring the accuracy of every antibody sequencing result. Our platform is suitable for different antibody subtypes such as IgA, IgE, IgG, and IgM, as well as various forms of antibodies, including fluorescent-conjugated antibodies, immobilized antibodies, and antibody sequence analysis from different species.

In addition, our team boasts a mature high-throughput Expression Verification Platform capable of high-throughput expression verification for the sequenced antibody, ensuring the activity of the delivered antibodies. With a well-established Antibody and Protein Platform, we can offer a one-stop service from Antigen to Antibody Sequencing, Antibody Gene Cloning, Antibody Chimeric Assembly, Recombinant Antibody Prodcution, ELISA Assay Development, and even Large-Scale Production Services, saving you valuable time.

Project Features

-- Only 200 µg of antibody is required for LC-MS/MS analysis.

-- De novo protein sequencing technology based on protein databases.

-- Applicable to antibodies from different species and different antibody subtypes.

-- Direct quantification of post-translational modifications and variant sequences.

Main Workflow of Antibody Sequencing

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Applications of Antibody Sequencing

Obtaining the gene sequence of monoclonal antibodies through antibody sequencing services and recombinant expression of the target antibody ensures stability and effectively avoids endogenous biological contamination. Antibody sequencing has broad applications, including:

Antibody Drug Development

* Accurate primary structure information is essential for drug development. Antibody sequencing analysis provides the foundation for the development of antibody drugs.

Antibody Engineering Research

* The obtained antibody sequences can be used for recombinant expression to produce various forms of recombinant antibodies and enable antibody humanization.

Patent Application

* Provides authorized monoclonal antibody sequence information for the application of protective patents.

Service advantages

Antibody Sequencing Service Accuracy Guarantee

* We implement strict quality control throughout the process, using the "wide in, narrow out" principle, ensuring the accurate identification of antibody sequences without missing valid data.

* The laboratory uses the Orbitrap Fusion Lumos Mass Spectrometer, which, compared to the previous generation Orbitrap Elite, increases the speed of primary and secondary mass spectrometry scans while ensuring the accuracy of peptide molecular weights.

* Combining our logical algorithms and software's big data processing capability (based on PEAKS software), our Antibody Sequencing Platform can distinguish amino acid mass differences that are difficult for traditional techniques. For example, it can accurately analyze the mass difference of 0.03 Da between glutamine and lysine.

Antibody Sequencing Service Coverage Guarantee

* Depending on project needs, we use up to 8 different proteases to digest the target antibody, and utilizes PEAKS software to automatically achieve accurate sequence stitching of different light and heavy chain peptide segments.

* For Complementarity-Determining Regions (CDR) of the antibody, we generate peptide sequences with a single amino acid site 10 times, ensuring high coverage and accurate sequence stitching.

Post-Translational Modification Analysis Guarantee

* Our Antibody Sequencing Platform effectively distinguishes different variants of homologous amino acids. It can accurately determine leucine and isoleucine differences based on secondary fragmentation peaks.

* For post-translational modifications of amino acid molecules, our system can also distinguish them accurately.

Can Be Used for Fluorescently Labeled Monoclonal Antibodies, IgMs, and Other Non-traditional Antibodies Sequencing

* Our antibody sequencing technology can also accurately sequence antibodies labeled with small groups like FITC, Biotin, Alexa, and larger protein groups like GST, especially when increasing the antibody sample size.

* Our technology is applicable to various antibody types. We have successfully sequenced antibodies derived from mouse, rabbit, camel, hamster, and goat.

Rigorous Sequence Prediction and Strict Sequence Validation

* For each sequencing project, we provide a detailed experimental plan. We first predict the sequence of the project antibody. If the customer provides hybridomas, we can perform antibody sequencing and combine it with mRNA sequencing data to ensure the accuracy of each amino acid sequencing.

* We also establish a database based on the determined sequence and perform secondary alignment analysis of the collected mass spectrometry data to ensure the accuracy of the delivered product.

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