Leguminous plants are well known for their unique nodules and ability to perform biological nitrogen fixation, possessing significant economic and ecological value. Common leguminous plants include soybean, peanut, and alfalfa. Soybean, as a key economic crop, is rich in seed oil and protein, providing substantial nutritional and economic value. Alfalfa is a perennial Leguminous Plants characterized by high biomass yield, excellent nutritional quality, and broad adaptability.
KMD Bioscience has extensive experience in the genetic transformation of leguminous plants. For soybean transformation, both gene gun and Agrobacterium-mediated methods are available. In the gene gun method, shoot apical meristems or embryogenic suspension cells are used as explants and bombarded with the gene gun. In the Agrobacterium-mediated method, cotyledonary node axillary meristems are used as explants and infected with Agrobacterium carrying the target gene. Resistant buds are then obtained through antibiotic screening and further differentiated into transgenic lines.
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