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Custom Probe ServiceIntroduction

KMD Bioscience has been dedicated to molecular biology and cytogenetics research for many years. Our scientists have extensive experience and accumulated knowledge, enabling us to offer personalized solutions tailored to the specific needs of each customer's project. We develop various types of probes for Fluorescence In Situ Hybridization (FISH) and provide a full range of services from project planning, probe design and synthesis, hybridization detection, to final data analysis. With standardized procedures, a comprehensive technical service system, and a team of senior and meticulous research experts, we ensure to deliver high-quality and customizable FISH Services to support our customers' research.

FISH is a non-radioactive technique that combines molecular biology and cytogenetics. This method utilizes fluorescent probes that specifically bind to target sequences on chromosomes, enabling the visualization of specific locations on chromosomes. It helps us conduct gene-level research and analysis quickly, accurately, and efficiently. FISH is widely used in gene expression analysis, chromosomal structure and number anomaly detection, rapid clinical cancer diagnosis, prognostic markers, and human prenatal diagnosis, among other fields, offering immense practical value. The customized FISH Probe Services provided us are not limited to single gene or single region analysis. We offer customizable, labeled FISH probes for a variety of molecular biology and cytogenetics research projects.

FISH probe type

Types

Advantages

Double-stranded DNA Probe

Double-stranded DNA Probe

Single-stranded DNA (ssDNA) probes

* Stable, easier to use, more specific, resistant to RNase, better tissue penetration, no self-hybridization.

RNA Probe (Ribosomal Probe)

* Higher thermal stability, better tissue penetration, higher specificity, less affected by RNase.

Synthetic Oligonucleotide

* Economical, stable, highly specific, resistant to RNase, good tissue penetration, excellent reproducibility.

Based on the specific needs of the customer's project, we offer one-on-one consultations with our technical experts (please contact our experts or fill out the information registration form, and our technical support will contact you). 

Fluorophore labeling methods for probes

Indirect Labeling

Probes are labeled with biotin. After hybridization, detection is performed using fluorophore-conjugated avidin or streptavidin. Additionally, the fluorescence signal can be amplified using a streptavidin–biotin–fluorophore complex.

Direct Labeling

Fluorophores are covalently attached directly to the nucleotides or the sugar-phosphate backbone of the probe, or incorporated into the probe using nick translation with fluorescently labeled nucleoside triphosphates.

Customized service

We offers full-process consultation and customized services for your project:

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Probe Customization Process

Step

Timeline

Probe design and synthesis

1-2 weeks

Delivery: Customized FISH probes (RNA probes: 50T / DNA probes: 10T), FISH probes validation report (QC report)  and complete FISH experimental services (optional).


Service case


Service advantages

-- Provide personalized and customized services to meet specific customer needs.

-- Offer customized design services for probes larger than 150 kb, featuring high specificity and low error rates.

-- Capable of designing and synthesizing a wide range of probe types, including those for most plants, animals, and microorganisms.

-- Backed by an experienced team and mature technologies, our probes accurately and efficiently recognize specific binding sites, are stable, and can be stored long-term.

-- Provide FISH probe validation to ensure proper hybridization with the intended chromosomal region.

-- Deliver one-stop technical services, from project design and probe production to quality control.

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