-- Transformable varieties: By default, we use Nicotiana benthamiana for tobacco genetic transformation. Tomato varieties include MicroTom, Moneymaker, LA1781, and XinFan No. 2.

-- Customers may specify other varieties as needed or request T1 generation seeds. Please consult our technical staff for details.

-- Vectors: pCAMBIA series, pBin series, pGreen series.

-- Efficient experimental workflow, not restricted by experimental materials; aseptic seedlings are produced year-round in the laboratory, allowing experiments to be conducted at any time.

-- Equipped with a Gene Editing Platform for siRNA and gRNA sequence design.

-- Multiple types of promoter options, including constitutive (p35s, pUbi), inducible (pCAB), and tissue-specific (PDX1, pAP3).

-- Utilizes Agrobacterium-mediated transformation, which is rapid, efficient, stable, and primarily single-copy insertion. It supports various eukaryotic resistance markers such as hygromycin, G418, Basta, and kanamycin.

-- Seedling acclimatization services are available to support customers' subsequent research.

– Project traceability: Regular reporting ensures remote tracking of experimental progress and transparent project management.

1. What is the role of model plants in genetic transformation?

Answer: By cloning and analyzing the functions of genes in model plants, it is possible to understand how genes control physiological and developmental processes. Functional studies of model plant genes help reveal the molecular mechanisms underlying plant growth, development, and environmental responses. The research outcomes can be directly applied to crop improvement.

2. What is the principle of the Agrobacterium-mediated method?

Answer: The principle of Agrobacterium-mediated transformation is to utilize the transfer of the T-DNA region into the plant genome during Agrobacterium infection. The T-DNA on the Ti plasmid of Agrobacterium can stably integrate and express in plant cells.

3. What are the advantages and disadvantages of the gene gun?

Answer: Advantages: Suitable for a wide range of plants, including monocots and dicots; relatively simple operation; allows for rapid transformation results.

Disadvantages: May cause damage to the genome; transformation efficiency may be relatively low; requires specialized equipment.