Bovine monoclonal antibodies possess unique advantages in studying antigen structures. The ultra-long CDR3 loop of bovine antibodies is the longest among all species (comprising 50–61 amino acids), facilitating recognition of deeply buried epitopes. Structural and deep sequencing analyses have demonstrated that this ultra-long CDR3 loop contains an even number of cysteines, forming a distinctive structure where these cysteines contribute to a diverse disulfide bond "knob" domain supported by β-chain "hands." This elongated CDR3 loop enables bovine antibodies to more effectively target various bacteria and viruses or bind molecular targets, particularly antigens that are undetectable by conventional antibodies such as G protein-coupled receptors (GPCRs). Our independently designed experimental protocol allows for the production of bovine monoclonal antibodies with high purity and strong specificity.

    KMD Bioscience provides services for the preparation of bovine monoclonal antibodies based on phage display technology. We can also develop customized experimental protocols tailored to your specific needs, including the design of peptide antigens and the preparation of protein antigens.

--Complete information and raw data for all monoclonal antibodies' ultra-long CDR3H sequences

B cells derived from blood, lymphocytes, and spleen cells (>5×10^6 per sample); cDNA libraries, 3–4 vials per sample

--The constructed expression vector

High-purity fusion antibody (>90%)

– Experimental reports and raw data: including results from SDS-PAGE, ELISA assays, etc., with antibody validation results (e.g., Western blot, SPR) provided upon client request.

--pM-level affinity and high specificity

--Sequence Diversity

—Selection for validation of multiple antibody expressions

High cell fusion survival rate: Ensures experimental success.

--A stringent QC system and standardized operational procedures ensure reliable antibody quality delivery.

--Diverse antibody purification techniques and protocols: Meeting various purity and scale requirements.

– Diversified antigen immunization: Administering proteins, peptides, and various small-molecule antigens, providing end-to-end services ranging from antigen preparation to antibody production.

—Diverse antibody detection methods: Provides antibody quality assessment techniques such as ELISA, WB, SPR/BLI, enabling comprehensive evaluation of antibody quality.

--Comprehensive platform: Equipped with a mature antibody and protein production platform, offering multiple expression systems and various immunization routes, enabling simultaneous experiments to ensure high-quality monoclonal antibody production.

--Complete result analysis: Provide a comprehensive report detailing the experimental procedures.

Custom Services: Provide personalized customization to meet customers' actual needs.