Home
>>
Technical Platform
>>
Viral Vector and Cell Technology Platform
>>
Stable Cell Line Construction
Download Center
Quick Inquiry & Consultation
Stable Cell Line ConstructionIntroduction

KMD Bioscience has been engaged in protein expression research for many years and possesses extensive experience in developing stable cell lines. We provide high-quality Stable Cell Line Construction Services. Our technical team strictly controls every step of the experiment to effectively improve the productivity of stable cell lines, enhance experimental outcomes, and ensure product quality. The stable cell lines we produce are suitable for various research applications, including recombinant protein and antibody production, gene editing, and functional studies.

We have established a comprehensive Cell Biology Technology Platform and Standardized Cell Laboratories, as well as a complete cell bank resource. We can provide over 300 tumor and primary cell lines, all STR-authenticated. These cell lines cover nearly 30 types of cancers, such as lung cancer, small-cell lung cancer, cervical cancer, gastric cancer, liver cancer, colon cancer, osteosarcoma, glioblastoma, and neuroblastoma. Each cell line is strictly managed and validated, making them easily susceptible to lentiviral or adenoviral infection, which facilitates genetic manipulation and provides a wide variety of cell models for both research and industrial production.

Lentiviral Infection System

The lentiviral transduction system is a gene vector system developed based on HIV. It can infect both dividing and non-dividing cells and effectively integrate heterologous genes into the host chromosome, enabling stable gene expression with high safety. Our team utilizes a second-generation self-inactivating lentiviral vector system and has developed multiple gene manipulation vector systems. At the same time, we have established purification processes for lentivirus at various purity levels and are capable of packaging lentivirus according to different purity requirements. Our platform has extensive experience in lentiviral infection across various cell types and has formulated standardized workflows for constructing stable cell lines of different types, allowing us to deliver high-quality Stable Cell Line Construction Services.

Stable Cell Line Construction Services

We can design lentiviral vectors carrying target genes of different operation types according to customer requirements, including gene knockdown (KD), gene overexpression (OE), gene overexpression (Cas9-SAM), gene knockout (KO), and gene knock-in (KI). We also package and produce high-titer lentiviral particles (≥1E+8 IU/ml) at varying purity levels, infect target cells, and screen to generate corresponding stable cell lines to meet customers’ research or clinical experimental needs.

Type

Item

Technical Advantages

Gene Knockout

CRISPR/Cas9 Knockout Cell Line Construction Service

CRISPR/Cas9 system is currently one of the most widely used gene-editing technologies.

Gene editing is efficient, precise, and convenient to operate, enabling multiplex targeting modifications.

Gene Overexpression

IOS Overexpression Stable Cell Line Construction Service

Utilizes mature IOS recombination technology.

Site-specific integration rather than random integration.

It shows stable inheritance, and the genes are not lost during cell passaging.

* The integration sites are high-expression loci, ensuring high gene expression levels.

Integration sites are high-expression loci, ensuring high gene expression levels.- Multiple genes can be integrated simultaneously, enabling multi-gene co-expression.

Gene Silencing

IOS Silencing Stable Cell Line Construction Service

Utilizes mature IOS recombination technology.

shRNA is site-specifically integrated rather than randomly integrated.

shRNA is Stable inheritance and the target gene is not lost during cell passaging.

Gene silencing efficiency remains consistent during cell passaging.

Site-Specific Gene Integration

Precise Site-Specific Integration Cell Line Construction Service

Based on IOS technology, oligonucleotide recognition sequences targeting specific genes are used to guide conformational changes at the genomic target site.

Functional genes are inserted into the genomic target site via homologous recombination to achieve precise site-specific integration.

Service process

图片24.png

Technical Resources

Login

Don’t have an account?Sign Up Now

Register

Already have an account?Log In Now