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Antibody Labeling ServiceIntroduction

KMD Bioscience has been dedicated to research in the field of antibodies for many years. Antibodies and proteins can be crosslinked to various reporter enzymes, such as horseradish peroxidase (HRP) and alkaline phosphatase (AP), using different chemical conjugation methods to preserve the activity of the labeled antibody or enzyme. The labeled antibodies or proteins can be used for ELISA, Western blot, in situ hybridization, cell and tissue immunohistochemistry assays. With our extensive technical experience and comprehensive platform, we not only provide high-quality Antibody Production Services but also offer various high-quality Antibody Labeling Services. Labeled antibodies can be used for localization, qualitative, and quantitative analysis of the corresponding antigens.

We have established a complete labeling strategy and calculation scheme, ensuring that the enzyme/protein coupling ratio is close to 1:1, thereby preserving the activity of the label. Since antibodies have unique properties, we conduct quality control testing on the antibodies before starting the enzyme conjugation process. We offer a variety of tags and conjugates to choose from, and can label antibodies or proteins according to customer specifications. Additionally, we provide personalized solutions, selecting suitable labels or labeling methods, optimizing labeling processes to meet your specific needs.

Introduction to Antibody Labeling

After obtaining the target antibody by various methods, in certain cases, to facilitate the observation and detection of the antigen, we typically select easily measurable and highly sensitive substances as labels for antibody conjugation. These labeled antibodies use the amplification effects of the label to detect or quantify the antigen or antibody's properties and concentrations, manifested as color or spectral changes. By utilizing the unique physicochemical properties of the amino acids that compose the antibody, under specific conditions, the antibody binds to the label, forming an antibody label conjugate. Antibody labeling is mainly used for antigen localization analysis and can enhance detection sensitivity through the amplification effect of the label. It is a rapid, cost-effective method for quantitative determination.

Labeling Procedure Diagram

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Antibody Labeling Services

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1. Antibody Fluorescent Dye Labeling Service

Unlike enzyme labels, fluorescent dyes are small molecular compounds. Our experienced scientists strictly follow quality control (QC) and quality assurance (QA) protocols to monitor every labeling project. The fluorescent dye labeling services we offer include:

Fluorescein Derivatives

Rhodamine Derivatives

Coumarin Derivatives

BODIPY Derivatives

Cascade Blue Derivatives

Lucifer Yellow Derivatives

Phycobiliprotein Derivatives

Organometalic Compound

FAM series

FITC

2. Antibody Enzyme Labeling Service

Antibody AP (Alkaline Phosphatase) Labeling Service

* Alkaline Phosphatase (AP) is a hydrolase enzyme commonly used for antibody labeling. It catalyzes the formation of a yellow substance from nitrophenol, which can be read at a wavelength of 405 nm. Its catalytic efficiency is much lower than that of horseradish peroxidase (HRP).

* Advantages: Stable catalytic kinetic reactions, linear accumulation of the formed substrate.

Antibody HRP (Horseradish Peroxidase) Labeling Service

* HRP-labeled antibodies can be widely used in ELISA, Western Blot, and Immunohistochemistry (IHC).

* In ELISA experiments, HRP-labeled detection antibodies are commonly used. They catalyze the formation of a blue-colored chemical substance, with high catalytic reaction efficiency. The color development reaction is usually completed in 10-30 minutes (AP: 2-4 hours).

β-Galactosidase Labeling

* β-Galactosidase is widely used in molecular biology enzyme reporter systems. β-Galactosidase-labeled antibodies are typically used in colorimetric assays and electrochemical enzyme-multiplied assay techniques (EMATs).

* Our system can effectively differentiate post-translational modified amino acid molecules.

Glucose Oxidase  Labeling

* Glucose Oxidase (GOx), derived from Aspergillus niger, has a monomeric molecular weight of 80 kDa. It catalyzes the conversion of glucose to hydrogen peroxide, though it is prone to forming dimeric forms.

* GOx-labeled conjugates are commonly used in food industry fermentation process detection and sensor production for diabetes diagnosis.

3. Antibody Biotin Labeling Service

Biotin is another commonly used biomolecule for antibody and protein labeling. It has high affinity for the tetrameric chain of avidin, including both avidin and streptavidin. Therefore, biotin is widely used in Western Blot, IHC, and ELISA applications.

Service advantages

-- Strict Quality Control System: Skilled experimental technicians with rich labeling experience and short delivery cycles. 

-- Comprehensive QC and QA Programs: We control every stage of production and offer dual quality assurance for your product.

-- Wide Selection of Tags and Conjugates: With a variety of labels and conjugates available, we provide the most suitable labeling services based on your specific needs.

-- Diverse Purification Techniques: In addition to dialysis, we have a variety of purification systems, including affinity chromatography, ion exchange columns, and hydrophobic interaction chromatography, allowing us to develop a tailored purification plan for your needs.

-- Comprehensive Platform Construction: Conjugated products undergo biochemical and biophysical analysis, with services like MALDI-TOF, ESI, LC-MS, SDS-PAGE, IHC, and enzymatic kinetics analysis to ensure high-quality product production.

-- Complete upstream and downstream services: We offer one-stop technical services ranging from antibody production, recombinant antibody expression, antibody sequencing, to subsequent antibody labeling and modifications, saving valuable time and costs for our customers.

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