Yeast expression vectors are typically selected based on the intended purpose of gene expression. These vectors can be categorized into intracellular and extracellular yeast expression vectors. Intracellular expression vectors include the pGAP series and pPIC3.5K, which allow the target protein to be expressed within the cell. This helps avoid excessive glycosylation by yeast and is suitable for expressing cytoplasmic or non-glycosylated proteins without disulfide bonds. Intracellular expression vectors generally enable high expression levels, but the subsequent purification process is relatively complex. A commonly used extracellular expression vector is pPIC9K, which enables extracellular secretion of the target protein, making purification relatively easier. In addition to offering a variety of Pichia pastoris vectors, we also provide Saccharomyces cerevisiae vectors. Combined with large-scale fermentation, we can meet industrial-level production needs.

The yeast expression system is one of the most economical and efficient eukaryotic protein expression systems, capable of achieving both intracellular and secretory expression. Its culture media are relatively inexpensive, the cultivation conditions are not stringent, and it is suitable for industrial-scale production. Similar to mammalian cell expression systems, the yeast expression system can perform modifications such as glycosylation, acylation, lipid modification, and phosphorylation, which help maintain the native conformation of the protein. This makes it suitable for the production of high-value protein materials that closely resemble their native forms. Using yeast expression systems, people have successfully expressed a wide range of proteins from various organisms, including bacteria, fungi, viruses, protozoa, plants, vertebrates, and humans. These proteins include enzymes, proteasomes, proteasome inhibitors, receptors, single-chain antibodies, antigens, regulatory proteins and other types of proteins. In the medical field, yeast expression systems have been successfully applied in the production of genetic engineering vaccines, genetic engineering drug production (such as antibody drugs and therapeutic proteins), protein function studies and others.