Microorganisms are a group of organisms with small sizes and simple structures, often invisible to the naked eye, including bacteria, fungi, protozoa, algae, viruses, and others. They are ubiquitous in nature. By applying genetic engineering to modify microbial genomes at specific loci, microorganisms can be improved, and gene functions can be studied.
Microbial gene knockout can be achieved using the λ-Red homologous recombination method and the CRISPR/Cas9 system. The λ-Red homologous recombination method uses the λ phage Red recombinase system to facilitate homologous recombination with only 40-60 bp of homologous sequences required for efficient recombination. Compared to the traditional λ-Red recombination system, CRISPR-Cas9 tools offer advantages such as simplicity, high efficiency, no need for screening markers, and easy identification of positive clones.
KMD Bioscience, leveraging its extensive experience in genetic engineering and the established Gene Editing Platform, can provide customers with microbial Gene Modification Solutions and Strain Construction Services. These include bacterial species such as E. coli, Klebsiella pneumoniae, Salmonella, Bacillus subtilis, as well as microorganisms like Saccharomyces cerevisiae, Pichia pastoris, and Candida albicans. In addition, we offer downstream services such as Protein Expression and Purification.
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