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Fermentation Process Development
Fermentation Process DevelopmentIntroduction

KMD Bioscience has been dedicated to protein expression and production research for many years. Proteins are fundamental substances of life, and with the rapid growth of the protein drug market and the in-depth study of proteomics, the demand for large-scale protein production has been continuously increasing. Our scientists have accumulated extensive experience in protein expression. The Protein Expression Platform integrates multiple recombinant protein expression systems, including prokaryotic and eukaryotic systems, and has successfully developed recombinant protein products for our customers, covering categories such as cytokines, enzymes, viral antigens, and human disease-related proteins.

We have established a comprehensive platform for large-scale protein fermentation and purification, providing customers with industrial-scale fermentation technology services to meet their needs for large-scale protein expression and production. We have mature ultra-high-density fermentation technology and are equipped with various types of fermenters and bioreactors to meet different production requirements. At the same time, based on multiple protein purification technologies and high-quality protein purification instruments such as Akta and BioRad Biological systems, we can provide large-scale purification services and achieve recombinant protein production ranging from milligrams to grams. Through self-modified expression vectors and optimized experimental processes, high yields can be achieved within a relatively short period. In addition, we design appropriate expression systems and fermentation culture solutions according to customers’ research needs.

High-density fermentation technology

High-density fermentation refers to the fermentation technology that maximizes biomass accumulation under specific culture conditions and systems, without affecting the accumulation of intracellular products, thereby obtaining more target products. It is an effective method to achieve high-yield recombinant protein production.

We have built a High-Density Fermentation Technology Platform capable of providing high-density fermentation services for adherent cells at different volumes. The culture density of human-derived adherent cells can reach up to 4×10cells/ml, which is suitable for high-yield protein expression fermentation involving viral transfection, achieving fermentation levels of 1 g/L. Using this platform, we have achieved a record recombinant antibody fermentation expression level of up to 4.7 g/L. In addition, with aerobic fermentation technology, we can provide High-Density Fermentation Services for Prokaryotic and Yeast Systems, with protein yields significantly higher than those of conventional prokaryotic fermentation.

Main Process of Fermentation Services

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Fermentation Services

Large-Scale Protein Fermentation Services

Prokaryotic Protein Expression System

Yeast Protein Expression System

Insect Protein Expression System

Mammalian Cell Protein Expression System

*Gene optimization

*Gene optimization

*Gene optimization

*Gene optimization

*Expression vector construction

*Expression vector construction

*Expression plasmid construction

*Expression plasmid construction

*Small-scale expression verification

*Screening of positive strains

*P1 virus production and small-scale expression tests

*Transient protein expression

*Optimization of expression conditions

*Screening of high-copy strains

*Expression optimization and testing

*Screening of stable cell lines

*Large-scale protein fermentation production

*Adaptation of engineered strains

*Large-scale virus production

*Optimization of culture conditions

*Large-scale protein purification

*Optimization of expression conditions

*Large-scale recombinant protein expression and purification

*Optimization of expression conditions


*Large-scale protein expression and purification


*Large-scale protein production and purification

Service advantages

-- Rigorous quality control system, skilled technical personnel, well-equipped animal production facilities, and short delivery cycles.

-- Extensive experience in recombinant protein preparation: successfully prepared thousands of recombinant proteins for customers, including recombinant antibodies, secretory proteins, transmembrane proteins, proteases, etc., with a high success rate.

-- Multiple fermentation equipment specifications: fermentation equipment ranging from 10 L to 500 L, meeting customer needs for different fermentation volumes and expression systems for research and production.

-- Equipped with GMP-like production workshops and standardized production management environments to ensure product quality.

-- Proprietary self-modified expression plasmids.

-- Downstream process development, optimization, and scale-up services compatible with fermentation processes.

-- Able to provide one-stop technical services from Protein Expression and Production to Large-Scale Protein Production, Purification, Lyophilization, and subsequent Characterization, saving valuable time and costs for customers.

-- Project traceability: Regular reporting ensures customers can remotely monitor experimental progress with full project transparency.

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