Bio-layer interferometry is a label-free, real-time optical detection technology primarily used for comprehensive quantitative analysis of biomolecular interactions and protein concentration measurements. This technique utilizes probe-based biosensors for direct sample detection. By monitoring the spectral shifts in interference patterns, it reveals the binding and dissociation dynamics between biomolecules interacting on the sensor surface. When a ligand binds to the end of the sensor, it forms a biological layer. Upon binding of the analyte to the ligand, the molecular weight at the sensor tip changes, leading to a change in the thickness of the biological layer. As light passes through this biological layer, it undergoes transmission and reflection to form interference waves. Changes in the layer’s thickness cause relative shifts in the interference pattern, which are displayed in real time as nanometer (nm) displacements on the interference spectrum.

Figure 2 BLI Principle Diagram

BLI technology can detect various sample types, including proteins, nucleic acids, polysaccharides, lipids, small-molecule drugs, antibodies, viruses, bacteria, and cells. It is now widely applied in life science research fields such as protein structure target analysis, drug development and screening, immunology, gene regulation, signaling pathways, genetics, microbiomics, virology, nanoparticle and liposome research, as well as analysis of traditional Chinese medicine extracts and natural products.

We provide label-free molecular interaction studies based on BLI: including protein–protein affinity measurement, antigen–antibody affinity measurement, protein–small-molecule drug affinity measurement; protein–DNA affinity measurement; antibody subtype analysis, antibody pairing, hybridoma screening, as well as affinity measurement of receptor-containing low-molecular-weight compounds and many other compounds, and high-throughput affinity ranking services according to customer requirements.

Depending on experimental objectives and sample types, we can select suitable sensors for each experiment. With more than ten types of sensors available, we are able to meet diverse sample requirements.

-- Affinity and kinetic measurements.

-- Screening of small-molecule compounds (antibodies).

-- Antibody characterization.

-- Consistency evaluation and quality control measurements.

-- Professional technical team with extensive experience.

-- High measurement efficiency, label-free and real-time monitoring.

-- Advanced instrumentation.

-- Broad application range: suitable for protein, nucleic acid, peptide, and nanomaterial interaction analysis.

-- One-stop service: providing integrated solutions from affinity measurement to molecular interaction studies.