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Peptide Library Screening ServiceIntroduction

KMD Bioscience has dedicated years to research in protein expression, production, and related fields. Our scientists provide comprehensive solutions for protein-related challenges. In the study of Protein Interactions, there is an urgent need for high-throughput screening services to meet the needs of a large number of researchers looking for protein or enzyme ligands. The Phage Display Peptide Library Screening Platform provided by KMD is a high-throughput peptide screening method. Through this technology platform, up to 1013/ml peptide or antibody clones that have been displayed by phage can be constructed, and the average number of independent clones can be achieved to 109-1010/ml. After multiple rounds of Peptide Library Screening, up to 105 effective peptide or antibody sequences can be found, which can provide a solid research foundation for the subsequent search for enzyme substrates, protein ligands, and highly specific antibody research. 

At the same time, with the Affinity Screening Platform of KMD Bioscience, the affinity ranking of different peptides or antibodies and target proteins can be obtained in a short cycle of time (2-3 days), and even the determination of precise affinity can be completed (See more in Affinity Determination Service). Based on the Phage Display Platform, KMD can provide Peptide Library Screening Services to fully address diverse customer requirements, including: random 7-mer Peptide Library, 12-mer Peptide Library and 15-mer library, etc.

KMD Bioscience is committed to providing high-quality Peptide Library Construction Services and can customize Peptide Libraries of various types and applications according to customers' needs. KMD's premade Peptide Libraries and customized Peptide Library Screening Platform provide beneficial tools for scientists. The application areas include Peptide Library Screening for drug dicovery, Peptide Screening for biomaker discovery, and Peptide Library Screening for vaccine development, Cell receptor Targeting Peptide Screening, Peptide Drug Development Customized Services, Cancer Treatment Peptide Screening, Protein Interaction Peptide Screening, Cell Signaling Pathway Research Peptide Screening, Functional Peptide Development Services, Bioinformatics-assisted Peptide Library Design, Specific Peptide Formulation Screening and Custom Production of Synthetic Peptide Libraries.

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Fig. 1 Principle and process of Phage Display Peptide Library

Phage Display Peptide Library Screening Workflow

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Figure 2. Phage Display Peptide Library

With rich experiences and well established Phage Display Technology Platform, KMD Bioscience can provide a wide range of customized peptide libraries with different lengths and characteristics to meet customers' needs. We offer specialized services for Customer Peptide Library Construction and Screening using T7 and M13 phage system (KMD provides Peptide/Antibody Library Screening Services).

Service Process

Service Items

Nucleotide Sequence Systhesis

Random Synthesis of Client-Specified Length Nucleotide Sequences

Polymerization and Digestion

Polymerization and Restriction Digestion of Synthetic DNA Sequences

Electroconversion Libraries Preparation

ER2738 Host Cell Preparation and Recombinant Plasmid Transformation in E. coli

Peptide Library Capacity and Diversity DetectionLibrary Capacity Assessment by bacterial colony counting, Diversity Evaluation through NGS-based sequencing
Phage-Displayed Peptide Library ConstructionPeptide Library Packaging via Phage Infection
Delivery

Shipping with ice packs


Phage Display Peptide Library Screening Workflow

Service Process

Service Content

Timeline

Antigen Quality Control

Antigen Purity Analysis by SDS-PAGE

1 week
Antibody Screening

Indentification of antigen-binding clones through 3-4 rounds of screening

2 weeks
Antibody Sequencing

Sequencing of positive clone

1 week
Antibody Quality Control

Expression of target antibody in E. coli followed by quality control by ELISA

1 week

Advantages of Phage Display Peptide Library Screening Services

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