Recombinant protein expression in insect cells involves the production of two generations of viruses, referred to as P1 and P2. The P1 virus is mainly used for preliminary protein expression experiments and amplification of the P2 virus, while the P2 virus is used for large-scale Protein Expression and Production Services. The insect cell protein expression system is suitable for recombinant expression of membrane proteins. Membrane proteins with up to four transmembrane domains can be effectively expressed and purified in insect cells. Purification of membrane proteins is a time-consuming and labor-intensive process. KMD Bioscience can provide customized membrane protein products with purity up to 95%.
The baculovirus expression system in insect cells is one of the eukaryotic expression systems, characterized by high exogenous gene expression levels, ease of expressing heterologous multimeric proteins, and high safety. Recombinantly expressed protein products have correct post-translational modifications and biological activity, such as disulfide bond formation, phosphorylation, glycosylation, etc. Compared with mammalian cell protein expression systems, proteins produced by the baculovirus insect expression system have a lower degree of glycosylation. However, the advantage of the insect protein expression system is that transfection using viruses is relatively simple, and the transfection system is easier to establish compared to transient transfection in mammalian cells. We have rich experience in recombinant protein insect expression (baculovirus expression) production and can provide you with high-purity transmembrane protein products. We also offer multiple scales of Protein Fermentation Services. Combined with our comprehensive Protein Purification Platform, we can deliver high-quality recombinant protein products to clients within a short time.
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