Gene knock-in (KI) refers to the precise insertion of a specific gene or DNA sequence into the genome, primarily achieved through homologous recombination and the CRISPR/Cas9 system. In homologous recombination–mediated methods, a DNA repair template containing homologous recombination arms must be constructed. For the CRISPR/Cas9 system, Cas9 first cleaves the target sequence to generate DSBs. Subsequently, a highly homologous DNA repair template is introduced. The cell activates HDR or NHEJ repair mechanisms, and the exogenous donor DNA template enables precise gene knock-in.
KMD Bioscience, leveraging years of experience in genetic engineering, has established a CRISPR/Cas9-based Gene Editing Platform that provides customers with Plant Gene Knock-In Vector Construction and Genetic Transformation Services. This includes sgRNA Design, knock-in vector construction, genetic transformation, screening, verification and other experimental steps. Additionally, the platform supports downstream experiments such as plant protein expression and protein-protein interaction studies.
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