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Rabbit Single B Cell mAb ProductionIntroduction

KMD Bioscience has dedicated years to antibody preparation research. We possess an experienced antibody development team, mature technical expertise, and a comprehensive range of antibody production equipment. This enables us to provide clients with high-quality Recombinant Monoclonal Antibody Preparation Services for various species, including rabbit, mouse, alpaca, sheep, and chicken.

Compared to mouse monoclonal antibodies, rabbit monoclonal antibodies offer distinct advantages: higher affinity and recognition of a broader range of epitopes. Consequently, they are widely utilized in research, diagnostics, and therapeutics. Rabbit monoclonal antibodies typically exhibit dissociation constants (KD) in the picomolar (pM) range, whereas most mouse monoclonal antibodies have KD values in the nanomolar or sub-nanomolar range. This signifies that rabbit monoclonal antibodies often possess approximately 100-fold higher affinity than their mouse counterparts. Furthermore, the rabbit immune system excels at recognizing subtle epitope discrepancy, generates highly specific monoclonal antibodies targeting these epitopes and owns wider epitope recognition coverage.


Advantages of Single B Cell Monoclonal Antibody Production

Category

Single B Cell Technology

Hybridoma Technology

Phage Display Technology

Antibody

Natural

Natural

Unnatural

Antibody Affinity

High

High

Medium

Druggability

High

High

Low

Turnaround Time

16–20 weeks; Optional rapid immunization

20–26 weeks; Optional rapid immunization

16–20 weeks; Optional rapid immunization

Antibody Gene Sequence

Obtain directly

Further sequencing required

Obtain directly

Antibody Diversity

High

Medium

Medium

Species Available for Screening

Unrestricted species

Mouse, Rabbit

Unrestricted species

Rabbit Single B Cell Technology for Antibody (Single B) Production

KMD Bioscience's Single B Cell Antibody Preparation (Single B) leverages Flow Cytometry Sorting Technology. It capitalizes on the principle that each B cell produces only one specific antibody type. Antibody genes are directly amplified from individual B cells, and specific antibodies are obtained through recombinant expression. Based on the Single B Cell Technology, antigen-specific B cells can be isolated directly from peripheral blood following animal immunization, and their antibody gene sequences cloned. The resulting rabbit monoclonal antibodies exhibit outstanding advantages, including high specificity, high activity, and high affinity.

To meet diverse client needs, KMD Bioscience provides a comprehensive One-Stop Technical Service encompasses Antigen Design, Synthesis and Modification, Animal Immunization, Single B Cell Enrichment and Screening, Expression and Purification, and Functional Validation.

Rabbit Single B Cell Monoclonal Antibody Production Service Workflow

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Service Content for Rabbit Monoclonal B Cell Antibody Production

Step

Service Content

Delivery Materials

Timeline

Isolation of Animal Immune PBMCs

* Immunize the rabbits 4-5 times
* ELISA titer determination
* Peripheral blood collection
* PBMC isolation

* ELISA test results
* Standard experimental record
* PBMC, 1×106 cells per 10 vials

4 to 10 weeks
(Either standard immunization or rapid immunization can be selected)

Fluorescence-Activated Cell Sorting

* PBMC Fluorescence-Activated Cell Sorting

* Standard experimental result record

1-2 business days

Single B Cell Culturing and Screening

* 5 to 20 plates of 96-well plates for single B-cell culture and Positive clone screening

* Positive clone screening results
* Standard experimental record

2-3 Weeks
(Additional validation methods are available)

Positive Clones Sequencing

* At least 20 positive clones underwent RT-PCR and sequencing

* RT-PCR results
* Original sequencing data
Specific sequence
* Standard experimental record

1 Weeks

Vector Construction for Antibody Expression and Purification

* Construction of mammalian expression vectors
* Cell transfection
* ELISA verification

* At least 3 positive clone sequences
* Expression vector
* Purified antibody: 500 Micrograms per positive clone
* ELISA test results
* Standard experimental report

5-6 Weeks
(Additional validation methods are available)

Sample Requirements

Customer Provides

Requirements

Recombinant Proteins

* ≥2.5 mg protein: Purity >85%, molecular weight >10 kDa. Soluble protein concentration: 0.5~5 mg/mL. For antigen affinity purification: Additional 10 mg recombinant protein required.

Peptides & Small Molecules

* ≥10 mg unconjugated peptide: Purity >90%, ≥10 amino acids. Requires conjugation with KLH, BSA, OVA, or other carrier protein.
* Small molecule: Requires pre-delivery structural evaluation.

Advantages of Rabbit Monoclonal B Cell Antibody Preparation Services

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