Currently, Agrobacterium-mediated shoot apex and callus methods are commonly used for cotton transformation. The shoot apex method utilizes the characteristic that shoot apices can differentiate into complete plants. In this method, shoot apices are used as explants and co-cultured with Agrobacterium. Regenerated seedlings can be formed in 2–3 months. This method offers advantages such as being genotype-independent and having a shorter transformation cycle, making it an ideal cotton transformation system. The callus method uses callus tissue as explants, which are infected with Agrobacterium carrying the target gene. After selection on medium containing Basta, cotton resistant shoots can be obtained.

-- Available varieties: R15 for the callus method. No variety limitation for the shoot apex transformation method.

-- Customers may specify other varieties as needed or request T1 generation seeds. Please consult our technical staff for details.

-- Vectors: pCAMBIA series, pBin series, pGreen series, pUC series.

-- Deliverables include positive plants, experimental data, images, and a standard genetic transformation report.

-- Efficient experimental workflow, unrestricted by plant material; aseptic seedlings of commonly used plants for genetic transformation are maintained year-round, enabling experiments to begin at any time.

-- Utilizes Agrobacterium-mediated transformation, which is rapid, efficient, stable, and primarily single-copy insertion. It supports various eukaryotic resistance markers such as hygromycin, G418, Basta, and kanamycin.

-- Seedling acclimatization services are available to support customers’ subsequent research.

-- Project traceability: Regular reporting ensures remote tracking of experimental progress and transparent project management.

1. What is cotton genetic transformation?

Answer: Cotton genetic transformation refers to introducing exogenous genes into cotton plants via biotechnological methods and ensuring their stable expression in the cotton genome, thereby producing cotton varieties with improved traits or novel functions. This process generally includes gene cloning, vector construction, genetic transformation, and screening/identification steps.

2. How are transgenic cotton plants identified?

PCR screening: Specific primers are used to perform PCR amplification on screened cotton plants, producing target bands of approximately 500 bp.

ELISA method: Detects exogenous protein expression in transgenic plants at the protein level, allowing exclusion of plants containing the CP4-EPSPS gene (PCR positive) but not expressing the target protein.

3. What are the main methods of cotton genetic transformation?

The main methods include Agrobacterium-mediated transformation, gene gun method, and pollen tube pathway method. Among these, Agrobacterium-mediated transformation is widely used due to its efficiency, stability, and ease of operation. This method involves infecting cotton recipient tissues with Agrobacterium carrying the target gene, integrating the exogenous gene into the cotton genome.