scFv (single-chain variable fragment) is currently the most commonly used recombinant antibody, with a molecular weight of approximately 25 kDa. It consists of the heavy chain variable region (VH) and light chain variable region (VL) covalently linked by a short flexible peptide. scFv offers key advantages including small size, high tissue penetration, and strong specificity. It can be produced at large scale in prokaryotic expression systems and is widely applied in the diagnosis and treatment of diseases involving viruses, bacteria, toxins, and tumor-associated antigens.
KMD Bioscience has established a mature and robust technology platform for scFv antibody discovery and engineering, supported by a well-equipped laboratory system. The platform enables the construction of scFv antibody libraries derived from naïve/immunized mouse and rabbit splenic B cells, hybridoma cells, and human B lymphocytes. To meet diverse customer needs, we offer a comprehensive one-stop technical service covering natural library construction, immune library construction, fully human synthetic library construction, high-throughput phage display screening, soluble expression and purification of scFv antibodies, affinity maturation, and humanization engineering. This integrated approach significantly shortens the antibody development timeline, reduces R&D costs, and provides high-quality technical support for scientific research.
0
