Hydrolases are enzymes that use water molecules to cleave substrate chemical bonds, widely participating in the degradation and transformation of biological macromolecules such as proteins, nucleic acids, polysaccharides, and lipids. They perform important physiological functions in digestion and absorption, intracellular material cycling, and metabolic waste clearance, and are important targets in the development of drugs for various metabolic diseases and anti-infective therapies.

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