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Bifunctional protein BirA, His & MBP Tag

Bifunctional protein BirA, His & MBP Tag

Product Number

PB110

Alias

Specifications
  • 50ug
  • 100ug
Product Description
Catalog NumberPB110
Molecular NameBirA
SpeciesE. coil
HostE. coli
Size50ug, 100ug
Purity>95%, determined by SDS-PAGE
FormulationLyophilized from sterile PBS, pH 7.4, and 0.01% Tween80 are added as protectants
Storage ConditionAliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping ConditionLyophilized powder will be shipped on at ambient temperature. Please contact our customer service to get specific information about the shipping condition of your order.
FAQ
  • Q1-双功能蛋白BirA的主要功能是什么?

    具有生物素连接酶活性,可将生物素标记到特定蛋白,同时参与生物素代谢调控。

  • Q2-His标签和MBP标签的作用分别是什么?

    His标签用于镍柱纯化,MBP标签可增强蛋白可溶性,并辅助通过直链淀粉树脂纯化。

  • Q3-该蛋白的应用领域有哪些?

    用于生物素化标记实验、蛋白质相互作用研究、体外酶活性分析及结构生物学研究。

  • Q4-保存时需要注意什么?

    建议分装后-80℃保存,避免反复冻融;短期使用可置于-20℃(含50%甘油缓冲液)。

  • Q5-实验前是否需要去除标签?

    取决于实验需求,MBP/His标签通常不影响酶活性,但若需天然结构可通过蛋白酶切除。

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