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H1N1 (A/California/04/2009) HA Protein, His Tag, Avi Tag

H1N1 (A/California/04/2009) HA Protein, His Tag, Avi Tag

Product Number

KMP4465

Alias

甲型流感病毒H1N1血凝素蛋白, H1N1 HA, Hemagglutinin Protein, H1N1 HA Protein

Specifications
  • 50ug
  • 100ug
  • 200ug
Product Description
Alias甲型流感病毒H1N1血凝素蛋白, H1N1 HA, Hemagglutinin Protein, H1N1 HA Protein
Catalog NumberKMP4465
Product DescriptionThe H1N1 (A/California/04/2009) HA Protein(KMP4465) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus ((A/California/04/2009) (H1N1)) hemagglutinin (translated amino acids of ACP41105.1) (Y108F, Met1-Gly519), termed as HA-specific B cell probe, was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Molecular NameH1N1 HA
SpeciesH1N1
HostInsect Cells
Size50ug, 100ug, 200ug
Purity>90% as determined by SDS-PAGE
PurificationAffinity purification
Endotoxin<1.0 EU/ug determined by the LAL method
Formulation20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
BackgroundInfluenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.
Storage ConditionAliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping ConditionIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

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