KMP4463
巴二氏病毒gH和gL的异二聚体蛋白, EBV gH & gL Heterodimer, gH & gL Heterodimer, EBV gH & gL Heterodimer Protein
| Alias | 巴二氏病毒gH和gL的异二聚体蛋白, EBV gH & gL Heterodimer, gH & gL Heterodimer, EBV gH & gL Heterodimer Protein |
| Catalog Number | KMP4463 |
| Product Description | The EBV (strain B95-8) gH & gL Heterodimer Protein(KMP4463) is produced in HEK293 Cells. A DNA sequence encoding the (Ser19-Leu682) of Epstein-Barr virus (EBV) (strain B95-8) gH (P03231) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 , A DNA sequence encoding the (Asn23-Gly137) of Epstein-Barr virus (EBV) (strain B95-8) gL (P03212) was fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified |
| Molecular Name | EBV gH & gL Heterodimer |
| Species | EBV |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >90% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0 |
| Background | Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
gH/gL复合体介导病毒与宿主细胞的膜融合，是疫苗和中和抗体开发的关键靶点。
His标签用于纯化，Flag标签便于检测；Flag可通过肠激酶切割去除（需在订购时注明）。
是的，作为ELISA抗原检测患者血清中的IgG/IgM抗体，特异性＞95%。
通过非还原SDS-PAGE和质谱分析，确认gH/gL以1:1比例稳定结合。
支持，提供佐剂（如CpG）配伍建议，已用于兔模型诱导中和抗体。
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