DENV Envelope Protein, His Tag
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Product Number
KMP4429
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Alias
登革热病毒包膜蛋白, DENV Envelope, E Protein, DENV Envelope Protein
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Specifications
- 50ug
- 100ug
- 200ug
Product Description
| Alias | 登革热病毒包膜蛋白, DENV Envelope, E Protein, DENV Envelope Protein |
| Catalog Number | KMP4429 |
| Product Description | The DENV Envelope Protein(KMP4429) is produced in HEK293 Cells. A DNA sequence encoding the Dengue virus (DENV)(type 1, strain US/Hawaii/1944) E (ACF49259.1) (Val580-Lys680) was expressed with a polyhistidine tag at the C-terminus. |
| Molecular Name | DENV Envelope |
| Species | DENV |
| Host | HEK293 Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >75% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 250mM NaCl, 50mM Tris, pH8.0 |
| Background | Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
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