Monoclonal Mouse Anti- Human IgG3 ,HRP
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Product Number
SA2299
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Alias
HRP标记小鼠抗人IgG3
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Specifications
- 1ml
Product Description
| Alias | HRP标记小鼠抗人IgG3 |
| Catalog Number | SA2299 |
| Size | 1ml |
| Immunogen | 人IgG3 |
FAQ
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Q1-该抗体是否识别IgG3铰链区特异性表位？
是的，靶向IgG3重链铰链区的独特序列，不与其他IgG亚型交叉反应。
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Q2-是否适用于检测IgG3型自身抗体（如抗核抗体）？
可以，需结合间接免疫荧光（IFA）或ELISA平台验证特异性。
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Q3-是否会与IgG3多聚体（如类风湿因子）非特异性结合？
可能因Fc段暴露增加背景，建议使用Fab片段特异性抗体或预吸附样本。
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Q4-推荐的稀释比例是多少？
ELISA常用1:2000-1:10000，WB为1:1000-1:5000，需根据标记物优化。
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Q5-如何避免IgG3在WB中的拖尾现象？
使用还原条件（含β-巯基乙醇）充分裂解二硫键，并优化凝胶浓度（如10% SDS-PAGE）。
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- Contact Us
- Phone：400-621-6806
- Mobile：13110936064（WeC
- E-mail：[email protected]
- Address：Room 108, Building 10, No. 6 Hechang Road, Wuqing Development Zone, Tianjin City
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WeChat
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Service Account
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400-621-6806 -
3599163699 -
Monday to Friday 9:00-18:00 -
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