KMP1847
Transforming Growth Factor (TGF), TGF beta 1, Transforming growth factor beta-1 proprotein, TGF beta 1, Human TGF beta 1 Protein, His Tag, TGF beta 1
| Alias | Transforming Growth Factor (TGF), TGF beta 1, Transforming growth factor beta-1 proprotein, TGF beta 1, Human TGF beta 1 Protein, His Tag, TGF beta 1 |
| Catalog Number | KMP1847 |
| Molecular Name | LAP, TGF beta 1 |
| Species | Human |
| Host | HEK293 Cells |
| Size | 20ug, 100ug, 500ug, 1mg |
| Purity | > 95% as determined by Tris-Bis PAGE, > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Background | Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex. |
| Application | SDS-PAGE, WB, ELISA |
| Tag | His tag |
| Biological Activity | Immobilized Human LAP (TGF beta 1), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.0ug/ml determined by ELISA. |
| Predicted N Terminus | His |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage Condition | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Sequence | LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALY NSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYM FFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNR LLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDIN GFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRH |
| Molecular Weight | The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result. |
| Uniprot ID | P01137 |
| Note | For Research Use Only! |
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