Rabbit Anti-Human AIF1 pAb
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Product Number
PA6031
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Alias
AIF1, AIF-1, IBA1, IRT-1, IRT1, Iba1
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Specifications
- 50ul
- 100ul
Product Description
| Alias | AIF1, AIF-1, IBA1, IRT-1, IRT1, Iba1 |
| Catalog Number | PA6031 |
| Molecular Name | AIF1 |
| Host | Rabbit |
| Size | 50ul, 100ul |
| Clonity | Polyclonal |
| Purification | Affinity Purification |
| Formulation | Supply in PBS with 0.02% sodium azide, 50% glycerol, pH 7.4. |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to a.a. 1-147 of human Iba1 (NP_001614.3). |
| Application | WB, IHC, IF |
| Storage Condition | Store the product under sterile conditions after opening at -20℃. Avoid freeze-thaw cycles. |
| Shipping Condition | Shipped on ice packs. |
| Product Declaration | 本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。 |
| Product Full Name | Rabbit Anti-Human AIF1 Polyclonal Antibody |
FAQ
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Q1-AIF1在细胞凋亡中的作用机制是什么？
AIF1（凋亡诱导因子1）通过线粒体释放并转位至细胞核，触发Caspase非依赖性DNA断裂和凋亡。
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Q2-是否适用于区分凋亡类型（Caspase依赖与非依赖）？
可以，需结合Caspase抑制剂（如Z-VAD-FMK）处理样本，对比AIF1核转位情况。
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Q3-Western Blot中线粒体提取物如何处理？
使用线粒体分离试剂盒，添加蛋白酶抑制剂，避免反复冻融以维持AIF1完整性。
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Q4-是否与AMID或AIFM2交叉反应？
经测试无交叉反应，特异性针对AIF1。
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Q5-免疫荧光中如何增强核定位信号？
使用4%多聚甲醛固定，透膜处理（0.2% Triton X-100，10分钟），并延长一抗孵育（4℃过夜）。
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- Address：Room 108, Building 10, No. 6 Hechang Road, Wuqing Development Zone, Tianjin City
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Service Account
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400-621-6806 -
3599163699 -
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