Rabbit Anti-Human AKT1 pAb
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Product Number
PA5305
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Alias
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT1
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Specifications
- 50ul
- 100ul
Product Description
| Alias | AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT1 |
| Catalog Number | PA5305 |
| Molecular Name | AKT1 |
| Host | Rabbit |
| Size | 50ul, 100ul |
| Clonity | Polyclonal |
| Purification | Affinity Purification |
| Formulation | Supply in PBS with 0.02% sodium azide, 50% glycerol, pH 7.4. |
| Immunogen | A synthetic peptide corresponding to a sequence within a.a. 400 to the C-terminus of human AKT1 (NP_005154.2). |
| Application | WB, IHC, IF, IP |
| Storage Condition | Store the product under sterile conditions after opening at -20℃. Avoid freeze-thaw cycles. |
| Shipping Condition | Shipped on ice packs. |
| Product Declaration | 本产品仅用于研究用途(RUO)。不能用于诊断或直接用于人体实验。 |
| Product Full Name | Rabbit Anti-Human AKT1 Polyclonal Antibody |
FAQ
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Q1-AKT1与AKT2的功能差异是什么？该抗体是否交叉反应？
AKT1主要调控细胞存活和增殖，而AKT2侧重代谢调控；该抗体特异性针对AKT1，与AKT2/AKT3无交叉反应。
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Q2-是否适用于检测磷酸化AKT1（如Thr308或Ser473）？
不能，此抗体检测总AKT1蛋白，需配合磷酸化特异性抗体（如p-AKT1 Ser473）分析激活状态。
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Q3-推荐用于哪种癌症研究模型？
适用于乳腺癌、胶质瘤等AKT1高表达肿瘤，建议使用PTEN缺失细胞系（如U87MG）作为阳性对照。
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Q4-免疫沉淀（IP）实验的优化建议？
使用温和裂解液（如NP-40），避免剧烈震荡，并预清除样本以减少非特异性结合。
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Q5-抗体在石蜡切片IHC中的信号弱怎么办？
采用高压热修复（pH 9.0 EDTA缓冲液），并延长一抗孵育时间（4℃过夜）。
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