Camelids (camels, llamas, and alpacas) possess an immune system distinct from other animals. Besides containing conventional IgG in their serum, they also produce VHH antibodies that lack light chains. VHH, with a size of only 13-15 kDa, offers advantages including small size, high stability, excellent solubility, rapid clearance from blood, and deep tissue penetration, exhibiting unique antigen-binding properties superior to conventional antibody fragments. Leveraging these advantages, VHH is widely applied in areas such as biosensing, affinity capture, protein crystallization, molecular imaging, and the diagnosis and treatment of diseases.

KMD Bioscience provides customized Phage Antibody Library Construction, Screening, Expression, and Antibody Purification Strategies tailored to specific client requirements. According to client needs, we immunize alpacas with various types of antigens to construct VHH Immune Antibody Libraries based on phage display technology. The VHH Immune Libraries we constructed exhibit high diversity capable of effectively generating VHHs with strong affinity for antigens. Additionally, our laboratory maintains Pre-made Natural Immune Libraries, eliminating the need for animal immunization and significantly shortening VHH preparation time. Furthermore, we can construct Synthetic Libraries and Semi-Synthetic Libraries based on VHH according to client specifications. We isolate peripheral blood mononuclear cells (PBMC) from alpacas, clone the obtained VHH genes into phage display vectors, and generate vast VHH Antibody Libraries through reverse transcription and polymerase chain reaction. Using the client's target antigen, this library undergoes multiple rounds of screening and enrichment to obtain the required VHH antibodies for research, diagnosis, or therapeutic applications.

KMD Bioscience possesses a comprehensive Recombinant Antibody Expression Platform. This platform enables VHH expression using different protein expression systems tailored to client requirements. The mammalian expression system serves as the primary choice for expressing human or other mammalian proteins and is the most commonly used in research. This system correctly and efficiently recognizes the synthesis, processing, and secretion signals of eukaryotic proteins. It produces proteins with structures and functions closely resembling natural proteins. Additionally, it performs essential post-translational modifications such as glycosylation, phosphorylation, and acetylation. The system features straightforward expression and purification processes while enabling easy transfection of recombinant plasmids. However, protein production using the mammalian expression system often suffers from low yields and long preparation cycles. KMD Bioscience has dedicated years to optimizing and researching mammalian expression systems. Consequently, we provide clients with high-yield and high-quality VHH Expression Services. We are highly proficient in utilizing HEK293 or CHO cell lines for both transient and stable expression of VHH proteins.

We can also utilize the E. coli prokaryotic expression system to prepare soluble proteins. We optimize expression conditions to prevent inclusion body formation and ensure optimal protein activity. Additionally, we maintain a mature Baculovirus/Insect Cell Expression System for the production of viral recombinant proteins from baculovirus-infected insect cells. This system enhances protein solubility, enables post-translational modifications, and facilitates simultaneous expression of multiple genes, yielding higher quantities of both intracellular and secreted proteins. Our Protein Expression Platform facilitates both small-scale and large-scale VHH production, addressing diverse client requirements. We offer Comprehensive Integrated VHH Services, encompassing screening, expression, and purification. Furthermore, we perform VHH characterization and functional validation according to client specifications to guarantee preparation quality.