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Guinea Pig Recombinant mAb Production Service
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Guinea Pig Recombinant mAb Production Service

KMD Bioscience has been dedicated to antibody research for many years. We possess experienced technical personnel for antibody preparation and have accumulated extensive expertise in recombinant antibody and protein production. This enables us to provide customers with high-quality antibody products and technical services. KMD Bioscience has established a mature Antibody Platform and a Phage Display Technology Platform. We can provide Recombinant Monoclonal Antibody Preparation Services for different species, including rabbit-derived, murine, camelid, ovine, avian, and shark-derived antibodies.

Guinea pigs serve as an ideal antibody source species for allergy and allergic reaction research, and are also important model organisms in studies of various infectious diseases. KMD Bioscience provides premium Guinea Pig Recombinant Monoclonal Antibody Preparation Services. By utilizing Phage Display Technology, we can produce monoclonal antibodies with high specificity, potency, and affinity for customers within a relatively short turnaround time. Relying on our well-established platforms, KMD Bioscience focuses on delivering comprehensive One-Stop Technical Services. These range from antigen design, synthesis, and modification, through animal immunization, Phage Library construction and screening, to downstream antibody modification and application validation, meeting diverse customer needs.

Preparation of Guinea Pig Monoclonal Antibodies

Traditional murine heterohybridoma technology (mouse-rat or mouse-guinea pig combinations) for monoclonal antibody production has a major drawback. The hybridoma cells can only undergo limited passaging for no more than 1 to 3 months before losing their monoclonal antibody secretion capability. Hybridomas prepared using Phage Library Technology can be preserved as antibody genes within phagemid vectors. Leveraging the strengths of different protein expression systems, such as utilizing Cell-Free Expression Systems, enables animal-free production of recombinant monoclonal antibodies with both high specificity and affinity.

Through years of technical accumulation, we can isolate antibody variable region genes (Vλ, Vκ, and VH) from diverse species. These genes are recombined and cloned into an antibody framework scaffold to construct full-length antibody libraries. Employing various screening strategies, such as the decreasing antigen concentration strategy, and combined with our Multi-Mode Protein Recombinant Expression Platform for recombinant antibody expression (yields can reach -5g/L), we provide customers with highly specific, stable, and high-affinity monoclonal recombinant antibodies.

Preparation Process of Guinea Pig Monoclonal Antibodies

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Guinea Pig Monoclonal Antibody Production Services

Step

Service Content

Deliverables

Timeline

Hapten Modification

Small Molecule Modification

* LC-MS Identification Results of Modified Small Molecule Derivatives.

3-4 weeks

Complete Antigen Preparation

OVA (Ovalbumin)

* SDS-PAGE, UV, or MALDI-TOF Identification Results of The Complete Antigen.

1 week

KLH (Keyhole Limpet Hemocyanin)

HSA (Human Serum Albumin)

HGG (Human Gamma Globulin)

Phage Antibody Library Preparation

Antigen Emulsification and Immunization of 2-3 Guinea Pigs (5 times).

* PCR Results, Immunization Assay Results (Including ELISA Titer and Specificity Determination Results), Experimental Records.

16-18 weeks

Collection of Splenocytes (or Peripheral Blood Lymphocytes), Isolation of Variable Region Genes.

Phagemid Construction, Transfection, Expression, and Library Construction.

Library Screening (Solid-phase Screening, Solution-phase Screening, etc.).

* Antibody Sequences, Library Screening Results, ELISA-positive Clone Identification Results, Experimental Report.

Recombinant Antibody Expression

Antibody Screening, Construction of Expression Plasmids, Eukaryotic or Prokaryotic Expression of Recombinant Antibodies.

* Recombinant Antibody, Expression Plasmid, Clone Strains, Experimental Report.

1-2 months


Service advantage

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Comparative Analysis of Monoclonal Antibodies Across Species

Type

Characteristics

Murine mAb

• Mature preparation techniques and well-established technology; commonly used model species;
• Low antigen requirement and cost-effective;
• Minimal batch-to-batch variation enabling rapid, continuous production.

Rabbit mAb

• Mature production system with relatively low preparation costs;
• Superior specificity, higher affinity and activity compared to murine antibodies;
• Broader antigen recognition capabilities.

Alpaca VHH

• HCAbs with broad antigenic epitope binding capabilities;
• Enhanced stability due to structural disulfide bonds;
• More amenable to humanization transformation.

Ovine mAb

• Large body size suitable for high-volume serum or antibody production;
• High antibody titer, excellent stability, favorable commercial properties, and strong applicability;
• Broad application scope, covering fields such as diagnostics and therapeutics.


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