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Rat Recombinant mAb Production Service
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Rat Recombinant mAb Production Service

KMD Bioscience has dedicated years to research in the antibody field. Obtaining effective and stable recombinant antibodies is fundamental and critical to antibody drug development. KMD Bioscience has established a comprehensive Phage Display Technology Platform capable of preparing recombinant monoclonal antibodies from various species, including rabbit, mouse, alpaca, and ovine. Our antibody team possesses proficient technical expertise in in vitro diagnostics and antibody/antigen R&D, with extensive experience in antibody selection and characterization, ensuring the delivery of high-quality recombinant antibodies.

Rat monoclonal antibodies typically exhibit minimal background cross-reactivity and offer advantages such as low cost and convenient production. KMD Bioscience can produce rat monoclonal antibodies with high specificity, high activity, and high affinity for clients within a relatively short turnround time. Utilizing our phage display technology platform, rat antibody genes are cloned into phagemid vectors, enabling scalable rat monoclonal antibody production when combined with various protein expression techniques.

We provide clients with one-stop Customized Antibody Services, encompassing peptide immunogen design and synthesis, protein recombinant expression, animal immunization, hybridoma preparation, and antibody purification. We also offer a series of downstream antibody validation and related services to meet diverse client requirements.

Rat Monoclonal Antibody Production Protocol

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Rat Monoclonal Antibody Production Service

Step

Service Content

Deliverables

Timeline

Hapten Modification

Small molecule modification

* Identification results of modified small molecule derivatives by LC-MS.

3-4 weeks

Complete Antigen Preparation

Ovalbumin (OVA)

* Identification results of complete antigen by SDS-PAGE, UV, or MALDI-TOF.

1 week

Keyhole Limpet Hemocyanin (KLH)

Human Serum Albumin (HSA)

Human γ-Globulin (HGG)

Phage Antibody Library Preparation

Antigen emulsification & immunization of 2-3 rats (5 times).

* PCR results, Immunoassay results (including ELISA titer & specificity), Experimental records.

16-18 weeks

Collection of spleen cells (or PBMCs) & isolation of variable region genes.

Phagemid construction, transfection and expression, library construction.

Library Screening (solid phase, solution phase, etc.).

* Antibody sequences, library screening results, ELISA positive clone identification results, experimental report.

Recombinant Antibody Expression

Antibody screening, expression plasmid construction, recombinant antibody expression (eukaryotic or prokaryotic).

* Recombinant antibody, expression plasmid, clone strain, experimental report.

1-2 months


Delivery Result

Deliverables

Eukaryotic expression cell lines secreting rat monoclonal antibodies.

Purified rat monoclonal antibodies demonstrating high specificity and high affinity.

Comprehensive experimental and characterization reports, including gene sequencing results, SDS-PAGE profiles, ELISA detection results, etc.


Service Advantage

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Comparison of monoclonal antibodies across different species

Type

Key Characteristics

Murine mAb

* Mature preparation techniques, commonly used model species.
* Low antigen requirement and cost-effective.
* Small batch-to-batch variation enabling rapid, continuous production.

Rabbit mAb

* Mature preparation system with relatively low cost.
* High specificity; exhibits higher affinity and activity compared to mouse antibodies.
* Broader antigen adaptability range.

Alpaca mAb

* Heavy-chain antibodies (VHHs) with broad epitope binding capability.
* Enhanced stability due to structural disulfide bonds.
* Easier humanization process.

Ovine mAb

* Large size suitable for large-scale serum or antibody production.
* High antibody titer, stability, excellent commercial viability, and good applicability.
* Wide application scope covering diagnostics, therapeutics, and more.


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