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Mouse Recombinant mAb Production Service

KMD Bioscience has dedicated years to antibody research. Recombinant antibodies developed using Phage Display Technology exhibit high specificity and find broad applications in therapeutics and diagnostics. We possess a highly experienced antibody preparation team with mature technical expertise in recombinant antibody customization and state-of-the-art production equipment, enabling us to provide recombinant antibodies in various formats.

Mouse monoclonal antibodies, as common monoclonal antibodies, are characterized by high specificity and minimal batch-to-batch variation. Leveraging our established Recombinant Protein Expression Platform and Antibody Preparation Technology Platform, KMD Bioscience offers customized Recombinant Monoclonal Antibody Services for various species, including mice. Equipped with integrated standard production lines and continuously optimized processes, we deliver high-yield, highly specific recombinant antibody proteins through rigorous quality assessments. Furthermore, KMD Bioscience is committed to providing comprehensive one-stop services, covering diverse technical aspects from antibody preparation to modification and affinity determination.

Production of Mouse Monoclonal Antibodies

KMD Bioscience offers two approaches for Mouse Monoclonal Antibody Preparation: one utilizes Phage Antibody Display Technology to generate recombinant monoclonal antibodies; the other employs cell fusion technology to establish mouse hybridoma cell lines.

In the Phage Display process for recombinant monoclonal antibodies, we clone murine antibody variable regions (Vλ, Vκ, and VH) into a phagemid system fused with the M13 phage pIII protein. Subsequently, the selected murine variable regions demonstrating high specificity and affinity are cloned into plasmid vectors containing the mouse antibody constant region framework. These constructs are then transiently expressed using our Recombinant Protein Expression Platform, achieving yields of up to 5g/L.

Mouse Monoclonal Antibody Production Protocol

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Mouse Recombinant Monoclonal Antibody Preparation Service

Step

Service Content

Deliverables

Timeline

Hapten Modification

Small molecule modification

* Identification results of modified small molecule derivatives by LC-MS.

3-4 weeks

Complete Antigen Preparation

Ovalbumin (OVA)

* Identification results of complete antigen by SDS-PAGE, UV, or MALDI-TOF.

1 week

Keyhole Limpet Hemocyanin (KLH)

Human Serum Albumin (HSA)

Human γ-Globulin (HGG)

Phage Antibody Library Preparation

Antigen emulsification & immunization of 2-3 rats (5 times).

* PCR results, Immunoassay results (including ELISA titer & specificity), Experimental records.

16-18 weeks

Collection of spleen cells (or PBMCs) & isolation of variable region genes.

Phagemid construction, transfection and expression, library construction.

Library Screening (solid phase, solution phase, etc.).

* Antibody sequences, library screening results, ELISA positive clone identification results, experimental report.

Recombinant Antibody Expression

Antibody screening, expression plasmid construction, recombinant antibody expression (eukaryotic or prokaryotic).

* Recombinant antibody, expression plasmid, clone strain, experimental report.

1-2 months


Service Advantage

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