Bovine monoclonal antibodies offer unique advantages in studying antigen structures. The ultra-long CDR3 loop of bovine antibodies is the longest CDR3 loop found among other species (50-61 amino acids), facilitating the recognition of deeply buried epitopes. Related structural and deep sequencing analyses reveal that the ultra-long CDR3 loop contains an even number of cysteines, forming a unique structure where cysteines participate in creating a diverse disulfide-bonded "knob" domain supported by a β-strand "stalk". This long CDR3 loop enables bovine antibodies to more effectively attack various bacteria and viruses or grasp molecular targets, especially antigens unrecognizable by conventional antibodies, such as GPCRs. Our independently designed experimental protocols maximize the acquisition of high-purity, highly specific bovine monoclonal antibodies.

KMD Bioscience provides Bovine Monoclonal Antibody Preparation Services based on phage display technology. We can also tailor personalized experimental protocols according to your requirements, such as peptide antigen design and protein antigen preparation.

Sequence information and raw data for the ultra-long CDR3H of all monoclonal antibodies.

--B cells (>5x10⁶ per project) from blood, lymph, and spleen cells; cDNA library, 3-4 vials per project.

--Completed expression vectors.

--High-purity fusion antibodies (>90%).

--Experimental reports and raw data: Including results from SDS-PAGE, ELISA tests, etc., and upon client request, antibody validation results such as Western Blot, SPR, etc.