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Ovine Recombinant mAb Production Service
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Ovine Recombinant mAb Production Service

KMD Bioscience has dedicated years to Antibody Preparation Research. We possess experienced antibody preparation technicians with substantial expertise and specialized skills. Combined with our mature antibody preparation industrial production line, we can provide customers with Customized Monoclonal Antibody Services for different species. Based on comprehensive platform systems including antibody and protein platforms, we cover upstream and downstream services for antibody production. We offer one-stop technical services encompassing antibody preparation, purification, sequencing, and affinity maturation.

Ovine are large hosts. Generally, larger animal size correlates with a higher probability of obtaining ultra-high-affinity antibodies. KMD Bioscience provides customers with high-quality Ovine Monoclonal Antibody Preparation Services. We screen for high-affinity antibodies using phage display technology. Our comprehensive protein expression platform enables high-level expression of recombinant antibodies. This enhances the potential for antibody engineering and performance optimization. We maintain consistent communication with customers throughout the project. Project updates are transparent to ensure delivered antibodies meet the required purity and quality standards.

Production of Goat Monoclonal Antibodies

Murine monoclonal antibodies typically exhibit affinities around 10-9 M. Gene engineering now enables monoclonal antibody preparation from diverse species. Mouse/Ovine heterohybridomas generated via heterohybridoma cell fusion technology can secrete antibodies with affinities reaching 10-13 M. A primary drawback is that heterohybridomas often lose antibody secretion capability during in vitro culture. Furthermore, their passage number is severely limited. Producing ovine monoclonal antibodies at the same scale costs 3 to 4 times more than using recombinant technology for hybridoma production.

KMD Bioscience has established a robust Phage Antibody Display Technology Platform. This allows us to produce highly specific and highly active ovine monoclonal antibodies for customers within a relatively short turnround time. Using this platform, ovine antibody genes are cloned into phagemid vectors. Combined with various protein expression techniques (prokaryotic or eukaryotic), we enable ovine monoclonal antibody production at multiple scales. Currently, we offer Recombinant Antibody Expression Fermentation Services achieving levels up to 5 g/L. This improves batch-to-batch consistency and stability of antibodies.

Ovine Monoclonal Antibody Production Workflow

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Ovine Monoclonal Antibody Production Service Overview

Step

Service Content

Deliverables

Timeline

Hapten Modification

Small molecule modification

* Identification results of modified small molecule derivatives by LC-MS

3-4 weeks

Complete Antigen Preparation

Ovalbumin (OVA)

* Identification results of complete antigen by SDS-PAGE, UV, or MALDI-TOF

1 week

Keyhole Limpet Hemocyanin (KLH)

Human Serum Albumin (HSA)

Human γ-Globulin (HGG)

Phage Antibody Library Preparation

Antigen emulsification & immunization of 1 ovine (5 times)

* PCR results, Immunoassay results (including ELISA titer & specificity), Experimental records

16-18 weeks

Collection of spleen cells (or PBMCs) & isolation of variable region genes

Phagemid construction, transfection and expression, library construction

Library Screening (solid phase, solution phase, etc.)

* Antibody sequences, library screening results, ELISA positive clone identification results, experimental report

Recombinant Antibody Expression

Antibody screening, expression plasmid construction, recombinant antibody expression (eukaryotic or prokaryotic)

* Recombinant antibody, expression plasmid, clone strain, experimental report

1-2 months

Sample Requirements

Customer Provides

Requirements

Recombinant Proteins

* ≥ 3.5 mg, Purity > 85%, Molecular weight > 10 kDa, Soluble protein concentration between 0.5~5 mg/mL. If antigen affinity purification required: provide ~15 mg additional protein

Peptides and Small Molecules

* Free peptide ≥ 15 mg, Peptide purity > 90%, Minimum 10 amino acids. Must be conjugated to KLH/BSA/OVA or other carrier protein; Small molecules require pre-evaluation of structure

Advantages of Goat Monoclonal Antibody Production Services

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Comparison of Monoclonal Antibodies Across Different Species

Type

Characteristics

Mouse mAb

* Mature preparation techniques, commonly used model species.
* Low antigen requirement and cost-effective.
* Small batch-to-batch variation enabling rapid, continuous production.

Rabbit mAb

* Mature preparation system with relatively low cost.
* High specificity; exhibits higher affinity and activity compared to mouse antibodies.
* Broader antigen adaptability range.

Alpaca mAb

* Heavy-chain antibodies (VHHs) with broad epitope binding capability.
* Enhanced stability due to structural disulfide bonds.
* Easier humanization process.

Ovine mAb

* Large size suitable for large-scale serum or antibody production.
* High antibody titer, stability, excellent commercial viability, and good applicability.
* Wide application scope covering diagnostics, therapeutics, and more.

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