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Donkey pAb Production Services

KMD Bioscience has been dedicated to antibody production research for many years. Our technicians have accumulated extensive expertise from numerous successful production projects. We strive to provide clients with high-quality antibodies, recombinant proteins, and related services. Leveraging well-established platform systems, including our Antibody Platform and Protein Platform, we offer integrated upstream and downstream services for antibody production. This enables us to provide comprehensive one-stop technical services encompassing Antibody Preparation, Antibody Purification, Antibody Sequencing, and Antibody Affinity Maturation.

Polyclonal antibodies are present in the serum of immunized animals and can be obtained through direct serum isolation. Due to their advantages—such as the capability to recognize multiple epitopes and elicit reactions like agglutination and precipitation—they are widely applied in immunological diagnostics. KMD Bioscience possesses an experienced team for Antibody Preparation, mature antibody production facilities, and advanced techniques. In addition to preparing polyclonal antibodies from commonly used laboratory species like rabbits and rodents, we also offer clients Donkey Polyclonal Antibody Preparation services.

Donkeys are large host animals, enabling the commercial-scale production of antibodies suitable for commercial and industrial applications. KMD Bioscience can utilize various antigens—including small molecules, peptides, modified peptides, proteins, fusion proteins, and modified proteins—for polyclonal antibody preparation. Leveraging extensive successful preparation experience, we ensure timely completion of experiments. We guarantee a positive serum and antibody ELISA titer of ≥ 1:50,000. For polyclonal antibodies prepared using protein antigens, we ensure positive results in Western blot detection.

Donkey Polyclonal Antibody Custom Service Procedure

Step

Service Content

Timeline

Animal Immunization

• Client provides antigen (small molecules/peptides/proteins acceptable; purity >85%, concentration >0.5 mg/mL, minimum 2 mg).

12-14 weeks

• Immunize one donkeys (up to multiple ones) with 5 immunizations; conduct real-time ELISA titer monitoring.

• Collect 1 mL pre-immune serum as negative control.

• Perform ELISA titer testing on post-immunization sera (3rd serum sample optionally deliverable to client).

• Deliver to client: 1 mL pre-immune serum + 250-500 mL immune serum with ELISA test report.

Long-term Bleeding

• Immunize with 0.5 mg antigen emulsified in 0.5 ml Freund's Incomplete Adjuvant (FIA).

2-3weeks/ period

• Bleed 100-150 ml every 2 weeks; monitor serum ELISA titer.

• Deliver products to client with complete records and reports.

Terminal Bleeding

• Euthanize donkey for whole blood collection.

1-2 weeks

• Deliver crude blood product or provide further purification per client requirements.

Purification

• Purify donkey polyclonal antisera using DEAE Purification (ion-exchange chromatography) or Affinity Chromatography.

1 week

• Deliver purified polyclonal antibodies.

Donkey Polyclonal Antibody Production Protocol

50-KMDBioscience-polyclonal-antibody-development-service-donkey.jpg

Service Advantage

51-KMDBioscience-polyclonal-antibody-development-service-donkey.jpg

Delivery Result

Deliverables

Requirement

Serum

Pre-immune donkey antiserum 1ml and Post-immune donkey antiserum 250-500 mL.

Purified Antibody

Donkey-derived antibody purified by Protein G/A, 20-40 mg.

Analysis Report

Includes immunogen sequence, SDS-PAGE electrophoresis map, expression clone sequencing report, titer detection ELISA results; Provides antibody validation results (e.g., Western Blot, IHC, IP, IF) based on customer requirements.

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