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Mouse mAb Production Services

KMD Bioscience has dedicated years to antibody development research. Monoclonal antibodies are produced by monoclonal hybridoma cells formed through the fusion of activated B lymphocytes with myeloma cells.Our team comprises experienced antibody development technicians who have accumulated extensive expertise and specialized skills. Combined with well-equipped antibody production facilities, we provide customized monoclonal antibody services for various species.

Mice are one of the common model organisms in biology and medicine. Murine monoclonal antibodies are widely utilized, offering advantages such as high specificity and the capability for rapid, large-scale, and continuous production.KMD Bioscience offers clients two distinct approaches for murine monoclonal antibody development:Preparation of recombinant monoclonal antibodies using Phage Display Technology and preparation of hybridoma cell lines using Cell Fusion Technology.Clients can provide antigens including small molecules, peptides, modified peptides, fusion proteins, and modified proteins for monoclonal antibody development.

Based on our established Antibody Platform system, we provide comprehensive end-to-end services spanning upstream and downstream processes, including Antigen Design, Synthesis and Modification, Aanimal Immunization, Phage Display Library Construction and Screening, as well as Downstream Antibody Modification and Application Validation. Simultaneously, we offer antigen preparation services covering DNA Synthesis, Plasmid Construction, Protein Expression, Protein Purification, Peptide Synthesis and Modification, Small Molecule Structural Analysis, and Conjugation to Carrier Proteins. Furthermore, our antibody team possesses extensive experience in developing IVD-grade antibodies and antigens, with deep expertise in antibody selection and characterization; to meet diverse client requirements, we provide personalized customization and screening solutions.

Production Process of Mouse Monoclonal Antibody Hybridomas

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Mouse Monoclonal Antibody Production Service Overview

Step

Service Content

Timeline

Animal Immunization

* Immunization of Five Mice with Five *Immunizations (including One Booster Immunization).

8-10 weeks

* Collect serum from 5 mice and measure antibody titers by ELISA.

* Provision of a Detailed Immunization Report.

Hybridoma Preparation

* Fusion of Splenocytes from mice meeting Titer Requirements (Protein/Virus >105; Peptide/Small Molecule >104) with Myeloma Cells.

8-12 weeks

* Limited Dilution of fused cells and Cloning in 96-Well Plates.

* HAT Selection of Hybridoma Cells.

* Selection of 1-10 Positive Clones post Immunogen Detection, followed by Subcloning and Expansion Culture in 96-Well Plates.

* ELISA Screening of Positive Subclones with Expansion Culture.

* Provision of 1ml Supernatant/Positive Clone to client for testing.

Ascites Production (Optional)

* Ascites Production using client-selected Hybridoma Cells in Two Immunized Mice.

4 weeks

* Ascites Characterization: ELISA Titer Detection.

* Protein A/G Purification: Ascites Purification.

* Provision of Purified Antibody (1-3 mg/clone).

* Provision of Experimental Report and Remaining Ascites Antibody.

Note: Cell Supernatant Purification is alternatively available.

Functional Validation (Optional)

ELISA, WB (Western Blot), IF (Immunofluorescence), FLAC (Flow Cytometry Antibody Capture), IHC (Immunohistochemistry), etc.

1-3 weeks

Sample Submission Requirements for Clients

Immunogen Type

Sample Submission Requirements

Protein

>2.5mg, Purity >85%, Concentration >0.5mg/ml, Molecular Weight >10kDa.

Peptide

KLH/OVA-Conjugated Peptide 10mg, Purity >90%; BSA-Conjugated Peptide 5mg, Purity >90%; Or Peptide (Free Peptide) 15mg, Purity >90%, KMD Conjugation (Optional).

Small Molecule

KLH/OVA-Conjugated Small Molecule 10mg, Purity >90%; BSA-Conjugated Small Molecule 5mg, Purity >90%; Or Small Molecule 15mg, Purity >90%, KMD Modification And Conjugation (Optional).

Delivery Results

Deliverables

3-5 Positive Clones.

Mouse monoclonal purified antibody with high specificity and affinity.

Experimental Reports: Including SDS-PAGE, ELISA assay results, and antibody validation results (e.g., Western Blot, IHC, IP, IF) as client requirements.

Advantages of Custom Mouse Monoclonal Antibody Services

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